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KMID : 0545120170270010161
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 1 p.161 ~ p.170
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Selection of Potential Virulence Factors Contributing to Streptococcus suis Serotype 2 Penetration into the Blood-Brain Barrier in an In Vitro Co-Culture Model
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Liu Hongtao
Zhu Seng
Sun Yingying
Li Na
Gu Jingmin
Sun Changjiang
Feng Xin
Han Wenyu
Jiang Jianxia
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Abstract
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Meningitis caused by Streptococcus suis serotype 2 (S. suis 2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential S. suis 2 virulence factors have been identified. This greatly hinders the progress of S. suis 2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library of S. suis 2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing to S. suis 2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis of S. suis 2 meningitis and potential targets for the development of drug therapies.
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KEYWORD
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Streptococcus suis serotype 2, meningitis, blood-brain barrier, phage display technology, virulence factor
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